TRAIL在病毒损伤胰岛β细胞中的作用

Effect of TNF-related apoptosis-inducing ligand on virus-induced apoptosis in a human islet β cell line

目的 :研究肿瘤坏死因子相关凋亡诱导配体 (TRAIL)在病毒诱导的人胰岛 β细胞损伤中的作用。 方法 :用Annexin -V法检测比较柯萨奇B病毒 (CVB)和风疹病毒 (RV)引起的人胰岛 β细胞系CM细胞凋亡 ,再用可溶性TRAIL受体和抗TRAIL单克隆抗体进行阻断实验。结果 :5噬斑形成单位 (PFU)CVB3和CVB4作用CM细胞 5h导致 30 %以上CM细胞凋亡 ;0 0 1PFUCVB3和CVB4作用CM细胞 2 4h导致超过 80 %CM细胞凋亡 ;10PFURV作用CM细胞 2 4h导致 2 5 %CM细胞凋亡 ;4种可溶性TRAIL受体以及抗TRAIL单克隆抗体对CVB所致细胞凋亡有阻断作用。结论 :CM细胞对CVB更为敏感 ,TRAIL参与CVB诱导的CM细胞凋亡。

AIM: To study role of TNF-related apoptosis-inducing ligand (TRAIL) in virus-induced apoptosis in a human islet β cell line, CM cell line. METHODS: Using annexin-V assay to compare Coxsackie virus B (CVB)-induced apoptosis with Rubella virus (RV)-induced apoptosis in a CM cell line; using four kinds of soluble receptors of TRAIL and anti-TRAIL McAb to block the virus-induced apoptosis. RESULTS: 5PFU of CVB3 and CVB4 contacted with CM cells for 5 hours , there were more than 30% apoptotic CM cells; 0.01PFU of CVB3 and CVB4 contacted with CM cells for 24 hours, there were more than 80% apoptotic CM cells; 10PFU of CVB3 and CVB4 contacted with CM cells for 24 hours, there were only less than 25% apoptotic CM cells; soluble receptors of TRAIL and anti-TRAIL McAb could block CVB-induced apoptosis. CONCLUSION: CM cell line is more sensitive to CVB and TRAIL plays a role in CVB-induced apoptosis.