蛋白质芯片在功能蛋白组学研究中的应用

Application of protein chip technology to functional proteomics

蛋白质芯片技术的应用使得对数以千计的蛋白质进行高通量、平行分析成为可能。蛋白质芯片是一种固定了保持天然活性的蛋白质微阵列,广义上,将能与蛋白质特异性结合的DNA和RNA、糖类、合成多肽,其他能够从复杂的混合物中特异性地捕获目的蛋白的小分子物质的微阵列也称为蛋白质芯片。蛋白质芯片可以检测感染性疾病、自身免疫性疾病及肿瘤等患者体内的疾病标志分子,还能研究蛋白质的功能、与其他蛋白质之间的相互作用、蛋白表达谱等蛋白质组学研究所涵盖的内容。本文将重点阐述蛋白质芯片在功能蛋白质组学研究中的应用进展。

s] Protein chip technology allows high throughput detection of thousands of proteins simultaneously in parallel manner. Protein chip composed of the microarrays of functional proteins, in more broad sense, microarrays immobilized ligands such as DNA, RNA, carbonhydride, peptide and other small molecules that can specifically bond to the target protein from protein complex can also be termed as protein chip. It has been applied to diagnoses of autoimmune diseases, infectious diseases and cancer by detection of disease related biomarker, and to analysis of antibody-antigen, protein-protein, protein-nucleic acid, protein-small molecule interactions, as well as enzyme-substrate interaction, all of which fall into the scope of proteomic research. This review will mainly discuss the application progress of this technology to functional proteomics.