F/10和G/11木聚糖酶家族的不同热稳定性机制

Different Thermostable Mechanisms of F/10 and G/11 Xylanase Family

应用生物信息学方法分析了木聚糖酶初级序列中20种氨基酸同其最适温度之间的关系,发现在F/10家族中有正相关作用的氨基酸是W,负相关作用的是 A,D,H,S;理论上最高最适温度是119 8 ℃在G/11家族中有正相关作用的氨基酸是L,D,P,Y,负相关作用的是 H;理论上最高最适温度为105 6 ℃.惊奇地发现在不同家族中 D起不同的作用,这只能从它们各自不同的蛋白质空间结构上进行解释.从初级序列基础上证明了木聚糖酶的不同蛋白质家族有不同的热稳定性机制.

In this article, bioinformatics method was used to analyze the relationship between primary sequence and the optimum temperature of xylanase. The contents of 20 residues were multivariatly regressed with the T_(opt) of xylanase, and it was found that, in F/10 the positively correlated residue is W and the negative residues are A, D, H, and S; the calculated maximum T_(opt) is 119.8 ℃. Whereas in G/11 the positively correlated residues are L, D, P, and Y, the negative residue is H; the calculated maximum T_(opt) is 105.6 ℃. The surprising finding of different effect of D can explained by structures of (α/β)_( 8) of F/10 family xylanase and β-jelly roll of G/11 family xylanase. It was demonstrated that different families of xylanase have inconsistent mechanisms thermostable.