摘要
利用溶剂萃取、薄层层析对大叶紫薇提取物中三萜化合物进行分离,通过DSC,UV,IR和HPLC/MS对分离产物中具有降血糖的成分3进行结构分析,结果表明:以氯仿:丙酮为展开剂,可在硅胶板中将大叶紫薇中三萜化合物有效分离.对分离出的成分3,DSC测定其熔点为283～287℃,焓值为43.727 J/g;UV最大吸收波长为206 nm;MS测定其相对分子质量为457;IR光谱符合乌苏果烷型结构.经分析,具有降血糖的成分3即为熊果酸.
Solvent-extraction and thin-layer chromatography were used to extract and separate triterpene from Lagerstroemia specious L. Structures of the composition #3 were identified by DSC、UV、 IR and HPLC/MS.The results showed that, triterpene can be efficiently separated with thin-layer chromatography using: acetone as the developer. The result also indicated that, the melting point, enthalypy,optimum absorbance wave lenghth, and molccular weight were 283~287 ℃,43.727 J/g, 206 nm, and 45%, respectively. IR spectrum fit that of ursane structure. The result showed compound #3 was ursolic acid.
出处
《无锡轻工大学学报（食品与生物技术）》
CSCD
北大核心
2005年第1期65-68,共4页
Journal of Wuxi University of Light Industry
关键词
大叶紫薇
熊果酸
分离
定性
Lagerstroemia specious L.
ursolic acid
isolation
identification
