摘要
鉴于在菌种选育工作中必须准确测定菌株产酸水平,要求有可行的测定方法,对简单易行的纸层析 分光光度法测定发酵液中L 异亮氨酸质量进行了系统的研究,研究发现:选用合适的显色剂、层析溶剂及洗脱剂的同时选择最佳吸收波长、最佳洗脱时间及适宜的点样量,可以提高该测定方法的准确度和精密度,从而在菌种筛选过程中准确地测定发酵液中L 异亮氨酸质量.
The level of amino acid production with mutant strains requires accurate and feasible measurement, The method of simple paper chromatography was studied systematically in this paper, to measure the content of L-Isoleucine in broth. The results could be summarized as follows: the accuracy and performance of the method depend on the appropriate selection of color reagent, solvent system and eluent solution, as well as the proper selection of the optimum absorb wavelength, suitable time of eluent, and L-Isoleucine does amount. The content of L-Isoleucine in broth can be measured using this method in the course of screening mutant stains.
出处
《无锡轻工大学学报（食品与生物技术）》
CSCD
北大核心
2005年第1期95-98,共4页
Journal of Wuxi University of Light Industry
关键词
纸层析法
L-异亮氨酸
显色剂
层析溶剂
最佳吸收波长
最佳洗脱时间
paper chromatography
L-Isoleucine
color reagent
solvent system
optimum absorb wavelength
suitable time of eluent
