六味地黄汤对皮质酮所致小鼠下丘脑-垂体-卵巢轴紊乱的调节作用及其拆方研究

Effects of Liuwei Dihuang Decoction and Its Decomposed Recipes on Hypothalamus-pituitary-ovary Axis in Corticosterone Administrated Mice

目的研究比较六味地黄汤全方(LW)及其拆方三补(地黄、山茱萸、山药)和三泻(茯苓、泽泻、牡丹皮)对下丘脑垂体卵巢(HPO)轴的调节作用。方法以肌肉注射外源性皮质酮(CORT)导致小鼠HPO轴功能紊乱为模型,用放射免疫分析法检测血清雌二醇浓度及下丘脑促性腺激素释放激素(GnRH)含量,用Westernblot检测垂体促黄体生成素(LH)水平。结果肌注CORT导致小鼠体重下降、卵巢重量减少,血清雌二醇、垂体LH及下丘脑GnRH水平下降,口服LW(5、10、15gkg)对CORT所致的血清雌二醇、垂体LH及下丘脑GnRH水平下降具有不同程度的改善作用。LW及大、中剂量(96、64gkg)三补对模型小鼠卵巢重量减轻的趋势无明显改善作用,而三泻及小剂量三补(32gkg)却使小鼠卵巢重量进一步下降,单独应用三补和三泻对CORT处理小鼠血清雌二醇和垂体LH水平无明显调节作用。结论外源性CORT导致小鼠HPO轴功能紊乱,LW对CORT所致的HPO轴功能紊乱具有改善作用,三补和三泻的作用不如LW,单独应用时存在产生不良反应的可能性。

Objective:To investigate the effects of Liuwei Dihuang decoction(LW) and its decomposed recipes on hypothalamus-pituitary-ovary(HPO) axis in corticosterone administrated mice.Methods:Radioimmunoasay(RIA) was used to test the Concentrations of hypothalamus gonadotropin-releasing hormone(GnRH)and serum estradiol,the western blot technique was employed to quantify the level of luteinizing hormone(LH) in the pituitary.Results:The body weights and ovary weights of CORT administrated mice were markedly decreased.Meanwhile,the concentrations of serum estradiol,pituitary LH and hypothalamal GnRH in CORT treated mice were significantly decreased.Oral administration of LW showed little effect on the body and ovary weights in CORT administrated mice,but showed significantly improving effects on the levels of serum estradiol,pituitary LH and hypothalamous GnRH.Oral administration of three purgative (5.4g/kg) decreased body weight of tested mice even further,while administration of three purgative (1.8,3.6,5.4g/kg) or three tonic(3.2g/kg) induced further decrease on ovary weight of tested mice.The decomposed recipes of LW are no longer effective in ameliorating hormone secretion of HPO axis.Conclusion:Administration of CORT induced malfunction of HPO axis,and LW improved or restored the disturbance of HPO axis in CORT treated mice.The decomposed recipes of LW are no longer effective in ameliorating malfunction of HPO axis, indicating that there were co-operations among components of LW.