补肾活血方对实验性精索静脉曲张大鼠睾丸组织SOD、NOS、MDA水平的影响

Effect of Bushen Huoxue Prescription on the Levels of SOD、NOS、MDA in the Testis of Rat with Varicocele

目的观察模型大鼠睾丸组织超氧化物歧化酶(SOD)、一氧化氮合酶(NOS)、丙二醛(MDA)水平,以及补肾活血方对模型大鼠睾丸组织SOD、NOS、MDA水平的影响,揭示补肾活血方治疗精索静脉曲张性不育的机理。方法将75只3月龄雄性SD大鼠先随机抽出15只为假手术组,其余60只大鼠采用肾静脉缩窄法建立精索静脉曲张的病理模型,造模完成后再随机均分为模型组、补肾活血方低剂量组、中剂量组、高剂量组。造模完成后48天开始给药,连续给药60天,取出左侧睾丸,匀浆,分别检测其SOD、NOS、MDA水平。结果模型组大鼠睾丸组织SOD水平显著下降,NOS、MDA水平显著上升,与假手术组比较有非常显著性差异(P<001);补肾活血方能显著提高模型大鼠睾丸组织SOD水平,降低NOS、MDA水平。结论自由基损害是精索静脉曲张引起不育的重要病理因素,补肾活血方能减少模型大鼠睾丸组织内自由基的生成,提高其抗自由基能力,这可能是补肾活血方治疗精索静脉曲张性不育的机制之一。

Objectives:Observing the levels of SOD、NOS、MDA in the testis of rats with varicocele,and the effect of Bu Shen Huo Xue Prescription on it,to investigate the mechanism of Bu Shen Huo Xue Prescription for male sterility with varicocele.Methods:Seventy-five Sprague-Dawley(SD) male rats were divided into five groups:control group,model group,Bu Shen Huo Xue Prescription low dose group,middle dose group,and high dose group.Experimental varicocele was created in the rats by partial ligation of left renal vein.Forty-eight days after establishing the model,medicine was given for sixty days.Then left testis was collected to measure the levels of SOD、NOS、MDA.Results:Marked decrease in the level of SOD and marked increase in the levels of NOS、MDA were found for the model(P<0.01).Bu Shen Huo Xue Prescription could increase the level of SOD and decrease the levels of NOS、MDA.Conclusions:The damage of free radicals may be the mechanism of varicocele causing male sterility.Bu Shen Huo Xue Prescription can decrease the creation of free radicals and inhibit the damage of free radicals,this might be the mechanism of Bu Shen Huo Xue Prescription for treating male sterility with varicocele.