三唑磷蓄积对日本4种组织超微结构的影响

Effects of Accumulated Triazopnios on Ultrastructure in 4 Tissues of Charybdis japonica

首先根据急性毒性试验确定了三唑磷对日本?的安全浓度为0.27mg/L,然后采用透射电镜技术研究了该浓度下三唑磷蓄积对日本?4种组织细胞(肌肉、鳃、肝胰脏和心脏)超微结构的影响。结果表明:三唑磷对肌肉损伤的主要靶部位是线粒体和肌原纤维,表现为线粒体内嵴肿胀、空泡化,线粒体溶解,肌原纤维断裂,明暗带不清楚;鳃细胞的损害表现为鳃丝水肿,细胞器溶解,角质层变薄,线粒体、内质网的肿胀、解体,细胞核不规则,核膜肿胀突起;肝胰脏细胞的主要损害特征为肝管微绒毛减少、线粒体水肿解体、内质网扩张、脂肪滴增加;心脏细胞的毒理变化为线粒体内嵴肿胀、瓦解,肌原纤维不规则,细胞核膜肿胀、突起,内质网溶解。鳃和肝胰脏中的变化要比肌肉和心脏明显。

In this paper,firstly,the acute toxicity of triazopnios with different density on Charybdis japonica was determined,and the safety concentration was 0.27mg/L.And secondly, effects of 0.27mg/L triazopnios accumulation on ultrastructure of cell in 4 tissues (heart,gill,muscle and hepatopancreas) were studied.The results showed that the main alteration in musculature and myocardial cell were both mitochondria and myofibril.Mitochondria showed swelling and a lot of vacuoles,and myofibril broke and losed contracted function of myocardium with irregular light and dark bands.Besides,swollen nuclear membrane and decreased endoplasmic reticulum were also found in those cells.The gill cells treated with 4 chemicals had an obvious ultrastructural variation.The parallel and ordered structure of muscle was destroyed, and endoplasmic reticulum was swollen up, dilated and vesiculated.Cristaes in mitochondria was swollen up,and disintegrated partially.Cuticle of gill fibre was denatured and dropped,and nuclear membrane was swollen up and partially dissolved.The ultrastructural damages to hepatopancreatic cell were mainly decreased microvilli,mitochondria, increased lipid droplets,decreased endoplasmic reticulum and disruption and vacuolization of nucleus and nuclear membrane.Changes in gill and hepatopancreas were much more than those of the two in heart and muscle.