微生物聚多糖PS-238合成条件的研究

Production of a Microbial Polysaccharides by Fermentation

一株产碱杆菌AlcaligenesspNX3可分泌一种新型高分子多糖PS238,该多糖具有耐高温、耐酸碱、耐盐等优良特性。碳源、氮源等培养基组分对该多糖产量有重要的影响。文中采用单因素的实验方法确定了发酵培养基的碳源、氮源分别为蔗糖和蛋白胨,然后采用中心复合设计法对培养基的主要成分进行了分析和优化。结果表明,碳源是极显著的因素,K2HPO4次之。最优发酵培养基配方为,蔗糖浓度49g/L,蛋白胨浓度65g/L,K2HPO42g/L,MgSO403g/L,根据预测的最优发酵水平的营养配比,产量由1078g/L提高至2285g/L。

This paper is about the study on the strain of Alcaligenes sp.NX-3 which could produce a new type of biopolymer PS-238. PS-238 has the excellent stability towards temperature,pH and salts. It belongs to the family of microbial polysaccharides with a wide range of industrial applications. Studies on the nutritional requirement for optimal polysaccharride production were carried out. The carbon source and nitrogen source were first investigated respectively. Then central complex design method was applied for the optimization of cultural medium. It was shown that carbon source was the most remarkable factor for the production of PS-238. The concentration of K_2HPO_4 was aslo very important. By using the optimized medium, the yield of PS-238 increased from 10.78?g/L to 22.85?g/L