RT-PCR克隆人血清白蛋白cDNA及其序列分析

Cloning and Sequencing of Human Serum Albumin cDNA

为了构建人血清白蛋白cDNA橡胶树乳管特异性表达载体,提取人工流产胎儿肝脏组织总RNA,采用RT-PCR法得到其改良cDNA,T/A测序后,采用NCBI BLASTn软件分析测得的序列及由此推导的氨基酸序列,与NCBI数据库中公布的HSA cDNA及氨基酸序列比较其同源率。结果表明,两者分别达到99.6%和99.5%。这为后期橡胶树乳管特异表达载体的构建和以转基因橡胶树来表达人血清白蛋白的研究打下了坚实的前期技术工作基础。

In order to construct the specific laticifer vector of HSA cDNA,total RNA was extracted from fetal liver and modified cDNA was obtained by RT-PCR.The sequence of cDNA was obtained after TA sequencing using NCBI BLASTn software,and the amino acid sequence was induced.The homology rate was 99.6 % and 99.5 % respectively compared with the published sequences in NCBI.The work is basic and crucial for construction and expression of HSA gene vector specific to Hevea brasiliensis laticifer.