分光光度法测定葡萄糖酸锌片中葡萄糖酸锌的含量

Determination of zinc gluconate in zinc gluconate tablets by spectrophotometry

探讨测定葡萄糖酸锌片中葡萄糖酸锌含量的方法。方法 根据葡萄糖酸锌在碱性条件下与锌试液生成蓝色化合物 ,在6 2 0nm处有吸收峰的特点 ,用可见分光光度法测定其含量。结果 葡萄糖酸锌在 4 2 3～ 1 2 7mg·L-1 范围内 ,吸收度与浓度呈良好的线性关系 (r=0 9999,n =5 )。平均回收率 99 5 % ,RSD为 0 4 %。结论 可见分光光度法简单、准确 。

AIM To establish a method for determination of zinc gluconate in zincgluconate tablets by spectrophotometry. METHODS The content of zinc gluconate can be determined by blue compound formed zinc gluconate with zincous reagent on the condition of alkality, which implied absorption move to 620nm. RESULTS The linear range of zinc gluconate was 4 23～12 70 mg·L -1 , r =0 9999. The average recovery was 99 5% with RSD of 0 4%. CONCLUSION The method is simple and accurate and can be used for quality control of pharmacy.