RP-HPLC法同时测定人血浆中5种镇静催眠药物浓度

Determination of five hypnotic drugs in serum by RP-HPLC

目的 建立血浆中硝西泮 (nitrazepam ,NZP)、艾司唑仑 (estazolam ,EZ)、阿普唑仑 (alprazolam ,AZ)、咪哒唑仑 (midazolam ,MZ)和地西泮 (diazepam ,DZP) 5种镇静催眠药的HPLC检测方法。方法 采用日本分光JASCO 1 5 0 0系列高效液相色谱仪 ,KromasilC1 8柱 (1 5 0mm× 4 6mm ,5 μm) ,以泼尼松 (prednisone ,PDS)为内标 ,流动相组成为甲醇 乙睛 水 四甲基乙二胺 冰醋酸 (5 0∶5 0∶1 0 0∶2∶1 5 ) ,流速为 0 8ml·min-1 ,紫外检测波长 2 4 3nm ,灵敏度AuFs=0 0 2。结果 血浆中 5种组分和内标均能很好地分离 ,无明显的干扰峰。各组分线性良好 ,日内、日间RSD <5 %。结论 本法操作简便、结果可靠 。

Aim To develop a method for determination of nitrazepam(NZP), estazolam(EZ), alprazolam(AZ), midazolam(MZ) and diazepam(DZP) in serum by high performance liuquid chromatography(HPLC) for providing a scientific basis for clinical use of hypnotic drugs. Methods 0 25 ml serum sample was extracted by Ether,dissolved by mobile phase. Kromasil C18(4 6 mm×150 mm,5 μm) column was used;mobile phase: methanol acetonitrile water C 6H 16 N 2 acetic acid (50∶50∶100∶2∶1 5),flow rate 0 8 ml·min -1 . prednisone(PDS) was the internal standard,Ultraviolet detection wavelength was 243 nm,AuFs=0 02. Results The liner was fine. The intra day and the inter day precision were both less than 5% respectively. Conclusion The method is rapid,sensitive and practical.