HPLC法测定穿心莲内酯滴丸中穿心莲内酯的含量

Determination of the andrographolide in andrographolide pill by HPLC

目的 探讨测定穿心莲内酯滴丸中穿心莲内酯含量的方法。方法 采用HPLC法 ,色谱条件为 :Hyersil C1 8柱 ,甲醇 :水 (5 5∶4 5 )为流动相 ,流速 1 0ml·min-1 ,检测波长 2 2 5nm。结果 穿心莲内酯在 2 1 936～ 1 97 4 2 4mg·L-1 浓度范围内与峰面积呈良好的线性关系 (r=0 9999) ,平均回收率 99 6 1 % ,RSD =0 94 %。结论 HPLC法简便、准确、可控 。

Aim HPLC method for the determination of andrographolide in andrographolide pill was established.Methods AHyper sill C 18 columm was used ,with the mobile phase of methanol water(55∶45).The flow rate was 1 ml·min -1 ,and the detection wavelength was 225 nm.Results The concentration of andrographolide was linearlyrelated to its peak area in the rang of 21 936～197 424 mg·L -1 ,and the average recovery was 99 61%,with RSD of 0 94%.Conclusion This method is simple and quick ,reproducible ,and can be used for the quantitative analysis.