膀胱移行细胞癌中COX-2的表达与其生物学行为的关系

Expression of Cyclooxygenase-2 in Bladder Transitional Cell Carcinoma and Its Relation to Biological Behavior of Bladder Cancer

【目的】探讨环氧化酶 2 (COX 2 )在膀胱移行细胞癌 (BTCC)中的表达及其与BTCC生物学行为的关系。【方法】应用逆转录聚合酶链反应 (RT PCR)检测 10例液氮保存的新鲜BTCC组织和 10例肾移植供体正常膀胱组织标本的COX 2mRNA表达情况 ;采用免疫组织化学方法 (Envision二步法 )对 72例膀胱移行细胞癌组织标本和 10例肾移植供体正常膀胱组织标本进行COX 2蛋白表达检测 ,并分析COX 2的表达强度与肿瘤各项病理参数之间的关系。【结果】RT PCR检测 10例液氮保存的新鲜膀胱癌组织COX 2mRNA均呈阳性表达 ,10例肾移植供体正常膀胱组织仅有 1例COX 2mRNA呈微弱阳性表达 ,余 9例阴性 ,两者差异有显著性 (P <0 .0 5 ) ;免疫组织化学显示正常膀胱组织中未见COX 2表达 ,但在BTCC组织中有不同程度的表达 ,COX 2的表达强度与肿瘤的分级、分期和复发有关。【结论】BTCC中COX 2的异常表达与肿瘤的分级、分期和复发等生物学行为有关 ,提示COX 2可能在BTCC的发生发展中起重要作用。

Objective To study the expression of cyclooxygenase-2(COX-2) in bladder transitional cell carcinoma(BTCC) and the relationship with biological behavior and its clinical significance.Reverse transcriptase polymerase chain reaction(RT-PCR) was applied to detect the mRNA expression of COX-2 in 10 frozen specimens from BTCC ,and 10 frozen specimens of normal bladder tissues obtained from renal transplantation donors.Immunohistochemistry method (Envision method ) was used to detect the expression of COX-2 in 72 cases of BTCC ,and 10 specimens of normal bladder tissues obtained from renal transplantation donors.Furthermore ,the relationship between COX-2 expression level and cancer pathologic parameters was analyzed.Using RT-PCR,all 10 frozen specimens from BTCC were shown to express COX-2mRNA,while only 1 of 10 frozen specimens of normal bladder tissues expressed COX-2mRNA,with significant difference.Using Immunohistochemistry method ,no marked expression of COX-2 was observed in the 10 specimens of normal bladder tissues obtained from renal transplantation donors, while COX-2 protein expression was postive in 72 cases of BTCC,The intensity of COX-2 expression was associated with tumor grade , stage and recurrence.[Conclusion]The abnormal expression of COX-2 in BTCC was closely correlated with the grade , stage and recurrence of tumors.This result suggests that COX-2 may play a key role in the development and progression of BTCC .