心肌标志物的组合检测在诊断缺血缺氧性脑病中的应用

Combined Detection of Myocardial Markers Applied for the Diagnosis of Ischemic-hypoxic Encephalopathy

【目的】探讨AST、LDH、CK、CK MB、HBDH、MYO心肌标志物的检测对缺血缺氧性脑病 (HIE)的诊断意义。【方法】对 39例HIE患儿及 4 7例正常新生儿的血清在AU 4 0 0全自动生化分析仪上进行AST、LDH、CK、CK MB、HBDH、MYO的测定并分析单项和多项组合检测的诊断阳性率。【结果】HIE组除AST、CK MB外LDH、CK、HBDH、MYO与对照组比较差异有显著性 (均P <0 .0 1) ,单项指标诊断阳性率在 6 4 .1%～ 79.5 %之间 ,四项组合阳性率达到 84 .6 %。【结论】多项心肌标志物的组合检测能协助临床快速诊断HIE。

Objective To explore the diagnostic significance of detecting six myocardial markers in ischemic- hypoxic encephalopathy (IHE).Serum samples were collected from 39 IHE newborns and 47 healthy newborns (control group). Serum activities of six myocardial markers in IHE and control groups were estimated by AU-400 system automatized biochemical analyzer, including aspartic acid aminotransferase(AST), lactic acid dehydrogenase(LDH), creatine kinase(CK),CK-MB, α-hydroxybutyric dehydrogenase(HBDH) and myokinase(MYO). The positive rate of diagnosis during single item and multiple items detection was analyzed.Except AST and CK-MB, serum activities of LDH, CK, HBDH and MYO in IHE group exhibited significant disparity as compared with those in control group (all P<0.01).The positive diagnostic rate of single item detection in this group was 64.1%～79.5%, while that of four items combined detection reached 84.6%.[Conclusion]Combined detection of multiple myocardial markers (AST, LDH, CK, HBDH and MYO) can help prompt clinical diagnosis of IHE.