小肠RNA对受照射小鼠粒系和巨核细胞系损伤的恢复作用

Restorative effect of intestinal RNA on injuries of granulocyte and megakaryocytic series in mice after abdominal γ-irradiation

目的 探讨小肠RNA在促进肠道辐射损伤恢复的同时对受照射小鼠粒系和巨核细胞系损伤的恢复作用。方法 BALB/c雄性小鼠接受 115 0cGy6 0 Coγ射线一次性腹部照射 ,于照射后1～ 3h内分别采用局部肠腔扩张注入法和肌肉注入法给受照射小鼠体内注入小肠RNA。照射后不同时间眼球取血 ,测定外周血细胞数变化 ;小平皿琼脂双层培养法测定股骨骨髓细胞CFU GM形成能力和脾 /体比。结果 (1)小鼠在接受一次性腹部照射后 ,除有肠道损伤外还存在着严重的造血损伤。其损伤在照射后 6h即以外周血WBC和PLT数减少 ,脾 /体比下降和骨髓CFU GM集落形成能力降低等形式表现出来 ,照后 1～ 8d最为严重 ,15d时开始恢复。 (2 )小肠RNA可促进受照射小鼠外周血WBC和PLT数的升高 ,提高脾 /体比 ,增强骨髓细胞CFU GM集落形成能力。 (3)小鼠受照射后一次注入小肠RNA即可起到很好的疗效 ,多次给药并不能增加疗效。结论 小肠RNA在促进肠道辐射损伤恢复的同时也能促进粒系、巨核细胞系损伤的恢复。

Objective To explore the restorative effect of intestinal RNA on injuries of granulocyte and megakaryocytic series in mice after abdominal γ-irradiation. Methods During 1-3 h after abdominal irradiation with 1150 cGy 60Co γ-rays,intestinal RNA was injected into gluteal muscle and regional intestine in mice. The changes of peripheral granulocytes and megakaryocytes cell CFU-GM(colony-forming unit-granulocyte/macrophage) and spleen weight index were measured at different times after irradiation. Results Besides small intestinal injuries there were also granulocyte and megakaryocytic series injuries after abdominal γ-irradiation. The numbers of leucocytes,platelets,CFU-GM and spleen weight index decreased at 6 h,were the most serious on 1 d to 8 d,and began to restore on 15 d after γ-irradiation.The intestinal RNA could increase the number of leucocytes,platelets,CFU-GM and spleen weight index after abdominal γ-irradiation. Single-injection had the same effect as multi-injection. Conclusion Intestional RNA can not only contribute to the restoration of the intestinal injuries but also enhance the restoration of granulocyte and megakaryocytic series injuries in mice after abdominal γ-irradiation.;