牡蛎酶解产物的ACE抑制活性和清除自由基活性研究

The ACE inhibiting activity and radical scavenging effects of oyster hydrolysates

研究了牡蛎蛋白质分别经537酸性蛋白酶和Alcalase碱性蛋白酶作用所得酶解产物的ACE抑制活性以及对DPPH·、·OH、O2·三种自由基的清除作用。结果表明,两种酶的作用产物都具有一定的ACE抑制活性和清除自由基活性。综合评价工艺条件以及功能实验结果得出,采用537酸性蛋白酶可以简化工艺,对于水解度为16％的酸性蛋白酶酶解产物,蛋白浓度为1mg/mL时,对ACE的抑制率为71.71％;20mg/mL时,对DPPH·、O2·自由基的清除率分别为76.23％、24.36％;8mg/mL时,对·OH自由基的清除率为8947％。相对分子质量分布结果表明,体系主要存在2肽到5肽的多肽类物质,HPLC图谱中对应峰面积约占总面积的76.99％。

The ACE inhibiting activity and radical(DPPH·, ·OH,O2·-)scavenging effects of oyster hydrolysates prepared with 537 acidic protease and alcalase were investigated. The results indicated that all hydrolysates had ACE inhibiting activities and radical scavenging effects. The application of 537 acidic protease could simplify the process for the production of bioactive peptides from oyster. For the hydrolysate with a DH of 16% prepared with 537 acidic protease, the inhibiting rate on ACE was 71.71% at a protein concentration of 1mg/ mL. The radical scavenging rates for DPPH·and O2·- were 76.23% and 24.36%, respectively at a protein concentration of 20mg/mL The radical scavenging rate for ·OH reached 89.47% at a protein concentration of 8mg/mL. The distribution of molecular weight measured by HPLC indicated that the peptides were mainly composed of 2-5 amino acid residues, and the corresponding peak area was 76.99% on the total area.