双载体固定化细胞发酵红曲色素的研究

On immobilized cell fermentation of monascus pigment with complex carriers

采用玉米芯为吸附载体,海藻酸钠为包埋剂对红曲霉细胞进行吸附包埋固定化培养。对以不同浓度的硝酸钾、大米粉、不同初始pH值等条件变化对细胞红曲霉菌进行发酵培养。通过正交优化实验表明:硝酸钾浓度为0 2%,米粉浓度为5%,培养基初始pH5 5时,通气量为75mL/500mL时产色素色价最高。包埋载体海藻酸钠的浓度为5%,固化剂氯化钙浓度为4%时,红曲霉的固定化粒子的机械强度较好。

The experiment of immobilized monascus purpureus for fermentation of monascus pigment were carried out by using the pith of maize as absorbing carrier and Na-alginate as entrapment agent. Fermentation for the cell monascus was carried out with different concentration of potassium nitrate,rice powder and preliminary pH. The optimizing experiment shows that the optimal conditions of fermentation are: KNO_3 0.2%, rice powder 5%, preliminary pH 5.5 and ventilation 75 mL/500 mL. When the Na-alginate's concentration is 5% and CaCl_2 is 4%, the strength of immobilized cell is best.