茵陈的HPLC指纹图谱研究

HPLC fingerprints of Herba Artemisiae scopariae

目的建立茵陈的HPLC指纹图谱,为茵陈的质量控制提供新方法。方法采用反相高效液相色谱法,KromasilODS色谱柱(250mm×4.6mm,5μm),流动相为1%醋酸水甲醇溶剂系统,线性梯度洗脱,检测波长326nm,柱温30℃,进样量5μL。以相似度和色谱指纹图谱指数鉴别药材质量,以指纹成分表观百分含量定量评价药材中各指纹成分含量高低。结果建立了茵陈药材的HPLC指纹图谱,以绿原酸峰为参照物峰,确定了22个共有峰,测定了不同产地茵陈HPLC指纹图谱与共有模式间的相似度。结论所建立的HPLC指纹图谱具有较好的精密度和重现性,适用于茵陈药材的质量控制。

AIM: A novel method for the overall quality control of Herba Artemisiae scopareiae has been established by HPLC fingerprint. METHODS: The chromatographic fingerprints were obtained by injecting ~5 μL of the sample solution each time on a Kromasil ODS column (250 mm×~4.6 mm ,~5 μm ) with the gradient elution solvent system composed of 1%acetate acid-water-methanol. The flow rate was 1 mL/min, the column temperature was maintained at ~30 ℃ and the detection wavelength was set at ~326 nm . RESULTS:22 co-possessing peaks were selected as the fingerprint peaks of Herba Artemisiae scopareiae and the similarities between the chromatographic fingerprints of the herbal drugs were calculated taking chlorogenic acid peak as the reference peak. The chromatographic fingerprints also were evaluated by the chromatographic fingerprint index (F), the relative index (Fr) and the fingerprint components apparent prercentage contents (P%). CONCLUSION: This method with good precision and reproducibility can be useful in the quality control of Herba Artemisiae scopareiae.