果胶酶的固定化

The Immobilization of Pectinase Encapsulated by Sodium Alginate

以海藻酸钠为载体固定化果胶酶 ,研究了海藻酸钠浓度、戊二醛浓度、CaCl2 浓度、酶使用量对固定化果胶酶活性的影响 ,同时对固定化果胶酶的特性进行了研究。结果表明 ,以 4 . 0 %的海藻酸钠为载体、0 . 2 0 %的戊二醛为交联剂、0. 10mol/L的CaCl2 为凝聚剂、用酶量为 0 . 5 0mL时所得的固定化果胶酶活力保存率可达95 . 2 % ,回收使用 10次以后 ,酶的活力回收率为 76 . 1%。

Sodium alginate was used as carrier for the immobilization of pectinase. The concentrations of carrier, cross linking agent, CaCl_2 and pectinase for pectinase immobilization were studied. The results showed that pectinase activity can retained up to 95.2% when prepared with 4.0% sodium alginate, 0.20% glutaric acid, 0.10 mol/L CaCl_2, 0.50 mL pectinase.The recovery activity of the immobilized pectinase was more than 76% after repeated 10 times.