气相色谱法分析奇蒿多糖的单糖组成及其含量

Analysis of the Monosaccharides in Artemisia anomala by GC

对奇蒿的水溶性多糖成分进行了分离提取及成分分析。采用浸泡、热提、醇析等方法提取奇蒿中的多糖成分 ;沉淀除蛋白质后 ,运用凝胶色谱法进行多糖纯化 ;经水解、衍生化后 ,用气相色谱方法分析其单糖的组成。结果表明 ,奇蒿多糖是由海藻糖、木糖、甘露糖、果糖等单糖组成。

Water-soluble polysaccharide was isolated and purified from Artemisia anomal using techniques of extraction precipiation, and purification. The components of the water-soluble polysaccharide were identified and quantified by capillary gas chromatography (CGC). The results showed that polysaccharide from Artemisia anomala contain fucose, xylise, mannose and fructose in the ratio 18.11∶30.12∶48.16∶1.99.