离子色谱法检测糖

Analysis of Carbohydrates by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD)

现有一种改进的色谱方法,用高效的阴离子交换柱分离糖,用脉冲积分安培检测器进行检测。它可以直接进行检测低浓度糖的定量分析,而无需衍生和样品的前处理。离子色谱的优点在于高pH条件下,糖能够很好的在阴离子交换柱上分离。以下将详细介绍此项技术。

An improved chromatographic technique known as high performance anion exchange (HPAE) was developedto separate carbohydrates. Coupled with pulsed amperometric detection (PAD), it permited direct quantification ofnonderivatized carbohydrates at low picomole levels with minimal sample preparation and clean-up. HPAE chromatographytook advantage of carbohydrates to give highly selective separations at high pH using a strong anion exchange stationary phase.These papers should be consulted for more specific details.