姬松茸液态发酵的研究

Studies on Liquid Culture Conditions of Agaricus blazei Murrill

通过2次L27(313)正交实验对姬松茸液态发酵的碳源、氮源、生长因子的种类和浓度、培养基的酸碱度、接种量、通气量、培养温度和培养时间等影响因子进行全面实验研究.经极差分析和方差分析,得到姬松茸液态发酵的培养基配方(g/dL):玉米淀粉3,豆粕0.8,麸皮0.15;发酵条件:pH5,接种量10%,摇瓶装量50mL培养基/250mL三角瓶,培养温度30℃,培养时间7d.在此培养条件下,姬松茸液体培养的生物量可达15.6g/L.

The influence of carbohydrate varieties and concentration, nitrogen varieties and concentration, growth factor varieties and concentration, pH, aeration volume, culture temperature, and culture time on liquid culture of Agaricus blazei Murrill(AbM) were studied by means of twice L_(27)(3^(13)) orthogonal tests. optimum formula for AbM in shaking flask culture was determined, which consist of 3 % of corn starch, 0.8 % of soybean cake meal, and 0.15 % of wheat bran, and its pH was 5.0. The optimum culture conditions are as follows, temperature 30 ℃, velocity of shaking table 120 r/min, fed volume 50 mL/250 mL and culture duration 7 days. Under above conditions, biomass of AbM attain to 15.6 g/L.