离子对高效液相色谱法测定阿昔洛韦脂质前药及前药的稳定性研究

Determination of lipidic prodrug of acyclovir by ion-pair high-performance liquid chromatography and its application to the study of prodrug′s stability

建立了同时测定阿昔洛韦脂质前药及其降解产物的离子对反相高效液相色谱法,并应用于前药稳定性研究。在体积分数85%甲醇流动相中添加十六烷基三甲基溴化铵,与前药降解中间产物形成离子对,使其与阿昔洛韦脂质前药和阿昔洛韦完全分离。前药在弱酸和中性环境中保持稳定,主要降解成阿昔洛韦。

Ion-pair high-performance liquid chromatography was used to determine the lipidic prodrug of acyclovir and its degradation products, which also was applied to the study of prodrug′s stability in buffers. The intermediate degradation product of prodrug combined cetyltrimethylammonium bromide to form ion-pairs with 85% methanol as the mobile phase, and the ion-pairs were separated from acyclovir and the lipidic prodrug of acyclovir on RP-HPLC. It was demonstrated from the study of stability that the lipidic prodrug kept stable in acidic or neutral buffer, and became unstable in basic buffer. The main degradation product of the lipidic prodrug was acyclovir.