原发性肝癌患者前S1蛋白检测与HBV-DNA的意义

Significance of Detecting Pre-S1 and HBV-DNA in Patients with PHC

目的了解前S1蛋白与HBVM、HBV-DNA检测的关系及其临床应用价值。方法用ELISA法检测146例PHC患者血清中HBsAg、HBsAb、HBeAg、HBeAb、HBcAb和前S1蛋白;同时采用荧光定量PCR法(FQ-PCR)定量检测其血清中HBV-DNA含量。结果146例PHC患者中HBV-DNA与前S1蛋白的阳性率分别为56.8%(83/146)和45.9%(67/146),两者阳性率差异无显著性;在HBVM各模式组中,小三阳组的病例最多占47.9%(70/146),前S1蛋白阳性率亦最高达67.1%(47/70),超过大三阳组57.1%(4/7)。结论前S1蛋白较HBeAg更敏感的反映了PHC患者体内HBV的复制情况,对HBV标志物及HBV-DNA的检测起着重要的补充作用。

Objective:To explore the relationship between hepatitis B markers (HBVM) and HBV-DNA with Pre-S1.Method:HBV-DNA concentration、Pre-S1 and HBVM (HBsAg、HBsAb、HBeAg、HBeAb、HBcAb) were detected by FQ-PCR(Fluorescence Quantitative PCR) and ELISA respectively in serum of the 146 patients of PHC.Results:In the 146 patients of PHC,the positive rates of HBV-DNA and Pre-S1 were 56.8%(83/146),45.9%(67/146),respectively.In all modes of HBVM,the case with HBsAg(+),HBeAb(+) and HBcAb(+) was 47.9%(70/146),and the positive rate of Pre-S1 was 67.1%(47/70);The case with HBsAg(+),HBeAg(+) and HBcAb(+) was 57.1%(4/7).Conclusion:This research showed that Pre-S1 was more sensitive than HBeAg in diagnosing viral replication in patients with PHC , and it could be used as a proper supplement to conventional test of HBV-DNA and HBVM.