液-液萃取RP-HPLC法测定人血浆中佐米曲普坦的浓度

Determination of Zolmitriptan in Human Plasma by RP-HPLC with Liquid-liquid Extraction

目的:建立RP-HPLC法测定人血浆中佐米曲普坦浓度。方法:以替硝唑为内标,血浆样品经液-液萃取前处理后,选用Hypersil C_(18)色谱柱(5μm,200mm×5.0mm),乙腈-磷酸盐缓冲液(0.05mol·L^(-1)磷酸二氢钠,用磷酸调pH至5.0)(15∶85)为流动相,室温下在223nm处进行测定。结果:测定佐米曲普坦血药浓度线性范围为1.92-66.14ng·mL^(-1),最低定量浓度1.92ng·mL^(-1);方法回收率为90.62%-105.7%,萃取回收率为71.70%-114.75%。结论:本法分离效果良好,灵敏度高,回收率高,日内、日间误差小,能满足于人体药代动力学及生物利用度研究。

Objective: To establish a RP-HPLC method for determination of zolmitriptan (ZOL) in human plasma. Methods: ZOL and internal standard (tinidazole, TDZ) were extracted from plasma with dichloromethane. The compounds were separated on a Hypersil C_(18) column(200 mm×5.0 mm,5μm). The mobile phase was a mixture of acetonitrile-0. 05 mol·mL^(-1)NaH_2PO_4 in a ratio of 15: 85 (adjusted pH to 5. 0 by H_3 PO_4), the flow rate was 1.0 mL min^(-1).The column temperature was room temperature. The detection wavelength was UV 223 nm. Results: A good linearity was obtained from 1. 92 to 66. 14 ng·mL^(-1)in plasma. The lowest concentration detected was 1.92 ng·mL^(-1).The recovery of method were from90.62%to 105.7%,and recovery of extraction were from 71.70%to 114.75%. Conclusion:The mrthod is sensitive,a accurate and reproducible with good recovery.