摘要
目的:研究灵芝及其制剂的指纹图谱的测定方法,确定灵芝HPLC指纹图谱。方法:ZORBAX×300SB-C_(18)色谱柱(4.6mm×250mm,5μm),MeOH-5%HAc(40∶60)溶剂系统等强度洗脱,检测波长252nm,流速0.8mL·min^(-1),进样量10μL,柱温40℃。结果:建立了灵芝及其制剂的指纹图谱。发现12个色谱峰为共有峰,确定了其相对保留时间的范围。结论:利用灵芝指纹图谱可以对灵芝制剂进行鉴别。该方法不需对样品进行任何前处理,快速简捷,精密度高,重现性和准确度良好,可为灵芝制剂的质量控制提供更为科学的依据和有效的鉴别方法。
Objective: To study the determination method of the fingerprint of lingzhi(Ganoderma lucidum) and its preparations and to establish the fingerprint of lingzhi and its preparations. Methods: The methanol extraction of lingzhi and its preparations was separated on a ZORBAX×300SB-C_(18) column(4. 6mm×250mm,5μm)elution using MeOH and 5%HAc-H_2O(40: 60) solvent system and detected at 252nm with a DAD detector. Flow rate was 0. 8mL·min^(-1) and 10μL was injected every time and the column temperature was 40℃. Results:The fingerprint of lingzhi and its preparations with 12 common peaks was established. The relative retention time was determined. Conclusion: The fingerprint can be used to distinguish lingzhi preparations. This method is quick, simple, repeatable, exact and need not any pretreatment. Therefore it provides scientific reference and effective determining ways for the quality control of lingzhi preparations.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2005年第3期352-355,共4页
Chinese Journal of Pharmaceutical Analysis
