摘要
目的探讨新型肿瘤坏死因子超家族成员TRAIL及共受体DcR4/DcR5在急性肾脏移植排斥反应作用。方法利用流式细胞术对正常人及发生急性肾脏移植排斥患者外周血单个核细胞(PBMC)表面的TRAIL分布进行探讨;用免疫组织化学的方法对正常人肾脏和急性排斥坏死肾脏病理切片DR4和DR5进行分析。结果正常人PBMC几乎不表达TRAIL,急性肾脏排斥患者PBMC高表达TRAIL,进一步证实其为IFN-γ+细胞表达;正常人肾脏表达的DR4和DR5低,但急性肾排患者高表达DR4和DR5。结论TRAIL及其受体DR4/DR5之间的作用可能参与了肾脏急性排斥反应。
Objective To explore the role played by TRAIL and its receptor DR4/DR5 in acute kidney transplantation rejection. Methods TRAIL on periphe ral blood mononuclear cells of normal person and patients with acute kidney tran splantation rejection syndrome were evaluated by flow cytometry. DR4 and DR5 on kidney of normal person and patients with acute kidney transplantation rejection syndrome were analyzed with immunohistochemistry(IHC). Results Nearly to TR AIL was found on normal human PBMC.In contrast, PBMC of patients with acute kidn ey transplantation rejection syndrome had high-expressed TRAIL. Further study in dicated that TRAIL-bearing cells were IFN-γ+ ones. Low density DR4/DR5 and high density DR4/DR5 were found on normal and patiets with transplanted kidney rejec tion,respectively. Conclusion Interaction of TRAIL and its receptor DR4/DR5 play important role in acute kidney transplantation rejection.
出处
《中国医学工程》
2005年第1期1-3,共3页
China Medical Engineering
