摘要
In the present study, immunohistochemical method (SABC method) was used to examine the distribution of serotonin-like neurons in hypothalamus of Cherry Valley ducks bred with high energy diet and low energy diet respectively. All films were analysed by using a computer-assisted image analysis system. In high energy group, labelled neurons are widely distributed in paraventricular nucleus(PVN, 17.73±1.41 neurons/19.46×103 μm2) and ventromedial hypothalamus (VMH, 15.8±1.71 neurons/19.46×103 μm2); in low energy group, labelled cells are widely distributed in lateral hypothalamus (LH, 11.25±1.53 neurons/19.46×103 μm2), yet there is no positive neuron in PVN. These results indicate that serotonin-like neurons in hypothalamus are involved in the regulation of food intake and energy metabolism.
In the present study, immunohistochemical method (SABC method) was used to examine the distribution of serotonin-like neurons in hypothalamus of Cherry Valley ducks bred with high energy diet and low energy diet respectively. All films were analysed by using a computer-assisted image analysis system. In high energy group, labelled neurons are widely distributed in paraventricular nucleus(PVN, 17.73±1.41 neurons/19.46×103 μm2) and ventromedial hypothalamus (VMH, 15.8±1.71 neurons/19.46×103 μm2); in low energy group, labelled cells are widely distributed in lateral hypothalamus (LH, 11.25±1.53 neurons/19.46×103 μm2), yet there is no positive neuron in PVN. These results indicate that serotonin-like neurons in hypothalamus are involved in the regulation of food intake and energy metabolism.
基金
the National Natural Sci.ence Foundation of China(39970547)
Hubei Natural Science Foundation of China(98J012).
