蛇毒蛋白C激活剂的活化蛋白C特性及影响因素的探讨

Research on the Protein C-Activating Property and Influencial Factors of Protein C Activator Isolated from the Venom of Agkistrodon halys

目的:研究蛇毒蛋白C激活剂(PCA-SV)活化蛋白C的特性,探讨可能影响PCA-SV作用的实验条件.方法:分别采用APTT法和CBS02.46发色底物法,研究PCA-SV的作用特点,探讨PCA-SV在不同实验条件下的抗凝活性及酰胺酶活性.结果:PCA-SV是蛋白C特异性激活剂,可特异性地作用于蛋白C,使后者表现抗凝和酰胺酶活性.PCA-SV的最适pH值为6.0～7.0,最适温度为37～40℃,肝素、枸橼酸钠等抗凝剂及Na+、K+、EDTA等不影响其活性.结论:PCA-SV是一种效价较高、特异性强、影响因素少的蛋白C活化剂.

Objective:To investigate the protein C activating property and experimental conditions that may affect the activities of protein C activator (PCA SV) Methods:PCA SV's functional characteristic,possible anticoagulant and amidase activities were measured by method of APTT and that of specific chromogenic substrate respectively in various experimental conditions Results:PCA SV can specifically act on protein C The pH optimum and temperature optimum for the action of PCA SV were 6 0~7 0 and 37~40℃ respectively. The anticoagulants (heparin, sodium citrate) had no effect on the activities Conclusion:PCA SV is a new protein C activating reagent that has high potency and specificity