海绵提取物S1～S3抑制肝癌细胞和肺腺癌细胞增殖作用的实验研究

Experimental Studies of Anti proliferative Effects of Marinesponge Extracts S_1～S_3 on Human Lung Adenocarcinoma and Hepatocarcinoma Cells

目的:用体外培养法研究海绵提取物S1～S3对肝癌7402细胞、肺腺癌GLC-82细胞和胃癌MCG-803细胞增殖的抑制作用.方法:采用MTT法进行研究.结果:海绵提取物S1、S2和S3分别处理肿瘤细胞48 h后,S3呈剂量依赖性抑制肺腺癌GLC-82和肝癌7402细胞增殖,测得IC50分别为2.714 μg/ml和2.598 μg/ml,而对胃癌MCG-803细胞增殖无明显抑制作用;S1和S2对3种肿瘤细胞增殖均无抑制作用.结论:海绵提取物S3对肝癌7402细胞和肺腺癌GLC-82细胞的增殖有抑制作用.

Objective: To study the anti proliferative effects of marinesponge extract S1～S3 on human lung adenocarcinoma GLC 82, hepatocarcinoma 7402 and gastric carcinoma MCG 803 cells in vitro Methods: The parameters were got by MTT assay Results: After human lung adenocarcinoma GLC 82, hepatocarcinoma 7402 and gastric carcinoma MCG 803 cells were treated by marinesponge extract S1～S3 for 48h, the growth of lung adenocarcinoma GLC 82 and hepatocarcinoma 7402 cells were inhibited significantly by the S3 and the IC 50 were 2 714 μg/ml and 2 598 μg/ml respectively; but no significant effect on the gastric carcinoma MCG 803 cells The marinesponge extracts S1 and S2 don't show inhibitive effect on the proliferation of those three tumor cells Conclusion: Marinesponge extract S3 can inhibit the proliferation of lung adenocarcinoma GLC 82 and hepatocarcinoma 7402 cells significantly