炒酸枣仁中酸枣仁皂苷的提取纯化工艺研究

Studies on the Extraction and Purification of Total Saponins from Parched Semen Ziziphi Spinosae

目的:建立从炒酸枣仁中提取酸枣仁皂苷的方法。方法:以酸枣仁皂苷A的含量和纯度为指标,采用正交设计优选酸枣仁皂苷的醇提工艺、大孔树脂纯化工艺。结果:酸枣仁皂苷的醇提最佳工艺为用 6倍量体积的80%乙醇回流提取 3次,每次 30min。HPD -100型大孔树脂纯化工艺为先用 150ml0 5% NaOH和 150ml30%乙醇依次洗去杂质,最后用 50ml70%的乙醇洗脱,酸枣仁皂苷A纯度可达 17. 9%,洗脱率达 72. 8%。

Objective:To study the extraction and purification process of the total saponin from Parched Semen Ziziphi Spinosae with ethanol and macroporous resin Method:The total saponins were extracted with ethanol and purified with macroporous resin by orthogonal design,taking content and purity of jujuboside A as guideline Results:The optimum extraction condition was adding 6 times amount of 80% ethanol and refluxing 3 times,for 30 minutes each time The purification process with macroporous resin HPD 100 was using 0 5% NaOH (150ml),30% ethanol (150ml) to wash out impurity,and 70% ethanol 50ml to wash out saponin The purity of jujuboside A was up to 17 9% and the eluted ratio 72 8%