茜素红S-铜(Ⅱ)金属配合物与溶菌酶作用研究

Study on Reaction of Alizarin Red S-Copper(II) Metal Complexes with L ysozyme

采用UV-Vis光谱法研究了pH=4.25的缓冲溶液中茜素红S(ARS)-铜(Ⅱ)金属配合物与溶菌酶(LYS)的结合反应。提出了双波长物质的量比法和平衡透析物质的量比法,与单波长物质的量比法进行对照测定研究,实验结果基本相符。研究发现,ARS-Cu(Ⅱ)-LYS的最大吸收波长为526nm,比ARS红移96nm,比ARS-Cu(Ⅱ)配合物红移10nm。在526nm处,测得ARS-Cu(Ⅱ)-LYS三元配合物的结合比为nARS︰nCu(Ⅱ)︰nLYS=6︰3︰1,摩尔吸光系数ε=7.93×104L·mol-1·cm-1,ARS-Cu髤配合物与LYS作用的条件平衡常数K=3.21×1012。ARS-Cu(Ⅱ)与LYS之间的作用力为配位键和电荷力。

The interaction of Alizarin Red S(ARS)-Copper(II) coordination compoun d with Lysozyme (LYS) was investigated by UV-Vis spectrophotometric method in ac idic buffer solution(pH=4.25). ARS-Cu(II)-LYS was a red color coordination compo und having a maximum absorption at 526 nm with 10 nm, 96 nm and 96 nm of red shi ft compared to the ARS-Cu(II) coordination compound, ARS complex and ARS-LYS com plex. Dual wavelength substantial amount ratio method, Balance dialysis substant ial amount ratio method and experimentation of unity wavelength substantial amou nt ratio method were suggested. The apparent substantial amount absorptivity of 7.93×104 L·mol-1·cm-1 was obtained. Combination proportion was defined as nAR S∶nCu(II)∶nLYS=6∶3∶1. Condition equilibrium constant K of ARS-Cu(II) and LYS was 3.21×1012. It is suggested that combined dint between LYS and ARS-Cu(II) w ere the coordinate bond and electrostatic force. Contemporary formation of elect ron transfer complexes is an importance factor in formation of ARS-Cu(II)-LYS Co mlexes.