摘要
The glucose oxidase (GOD) immobilized onto the surface of activated ca rbon powders at the glassy carbon electrode (GOD-C/GC) could undergo the quasi-r eversible, direct electrochemical reaction. Its formal redox potential, E0′, is almost independent on the scan rates. The average value of E0′ is (-0.467 ± 0 .002) V (vs SCE) in the pH 6.8 phosphate buffer solution. Its apparent heterogen eous electron transfer rate constant (ks) is (1.18 ± 0.59) s-1, which is much h igher than that reported previously. The dependence of E0′ on the pH of the buf fer solution indicated that the direct electrochemical reaction of the immobiliz ed GOD is a two-electron transfer reaction process coupled with two-proton trans fer. The further experimental results demonstrated that the immobilized GOD reta ined its bioelectrocatalytic activity to the oxidation of β-D(+) glucose.
The glucose oxidase (GOD) immobilized onto the surface of activated carbon powders at the glassy carbon electrode (GOD-C/GC) could undergo the quasi-reversible, direct electrochemical reaction. Its formal redox potential, E-0', is almost independent on the scan rates. The average value of E-0' is (-0.467 +/- 0.002) V (vs SCE) in the pH 6.8 phosphate buffer solution. Its apparent heterogeneous electron transfer rate constant (k(s)) is (1.18 +/- 0.59) s(-1), which is much higher than that reported previously. The dependence of E-0' on the pH of the buffer solution indicated that the direct electrochemical reaction of the immobilized GOD is a two-electron transfer reaction process coupled with two-proton transfer. The further experimental results demonstrated that the immobilized GOD retained its bioelectrocatalytic activity to the oxidation of beta-D(+) glucose.
出处
《无机化学学报》
SCIE
CAS
CSCD
北大核心
2005年第3期405-408,共4页
Chinese Journal of Inorganic Chemistry
基金
国家"211"工程重点学科建设和南京师范大学青年科学基金(No.2003XQ002)资助项目
关键词
GOD
直接电化学
葡萄糖氧化酶
固定
SCE
GC
direct electrochemistry
bioelectrocatalysis
glucose oxidase
activated carbon powder