摘要
对携带两个外源抗虫基因 (部分改造的BtCry1Ac基因和慈菇蛋白酶抑制剂基因API)的白杨杂种 74 1杨的抗虫性、生长特性、木材品质、结实特性及适应性进行多年连续观测。结果表明 ,3个转双抗虫基因 74 1杨株系对杨扇舟蛾、舞毒蛾、美国白蛾等鳞翅目食叶害虫具有高抗虫性 ,幼虫死亡率均在 80 %以上 ,并且还能抑制存活下来的昆虫幼虫的发育 ,使其发育速率减缓 ,不能正常结茧 ,连续 4年饲虫试验未发现其抗虫性有规律下降的趋势。转基因受体 74 1杨具有生长速度快 ,特别是胸径和材积生长在生长后期具有明显的速生优势 ,单株材积生长量平均超出对照 4 0 %以上 ;74 1杨木材品质优良 ,木材密度、纤维特性及各项力学性质均优于毛白杨。连续 3年观测表明 ,74 1杨为高度败育雌株 ,果穗小、数量少 ,成熟开裂前 70 %~ 90 %脱落 ,基本不飞絮。转基因 74 1杨没有发生明显变异 ,生长未受影响 ,可在毛白杨适生区推广应用。建议采用适宜的栽培模式 ,控制害虫抗性发展。
Years of series observation to the White Poplar Hybrid 741(Poplar 741) transformed two insect-resistant genes (BtCry1Ac and API genes rebuilded partly) in the character of the insect_resistance, growth, lumber quality, seeding character and the adaptability indicated that 3 of the Poplar 741 clones took on a high insect-resistant identity to the leaf pests such as the Clostera anachoreta Fabricius, and the Lymantria dispar Linnacus ect., and the mean death rate of the grubs were all above 80%, furthermore, the grubs' growth which survived could be restrained and the growth rate was reduced so that they couldn't tie cocoons as the usual. The quality of insect_resistantce of the Poplar 741 varieties had not taken on a rhythmical drop trend by 4 years tests. The giving body of the Poplar 741 varieties possesses the advantages of fast growing, and especially in the anaphase of the growth of the DBH, and the timber volume take on an evidently fast growth, the growth quantity of the timber volume per tree go beyond 40% compared with the control averagely. The Poplar 741 varieties have the fine lignum character and the mechanics character such as the lignum density and the fiber quality are all superior to the Chinese White Poplar. 3 years' series observation indicated that the two insect-resistant genes transformed Poplar 741 was the high yield trigamous plant and had the small, little spikes of which about 70%~90% fell off before they crazed when they were ripe and the Poplar 741 didn't fly garrulous basically. The Poplar 741 transformed genes could be extended in the proper area of the Chinese White Poplar under the condition that the Poplar 741 varieties haven't evident differentiation and the growth didn't get infection much. Taking feasible planting mode in order to control the development of the fastness of the pests should be considered.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2005年第1期91-97,共7页
Scientia Silvae Sinicae
基金
国家自然科学基金 (0 0 2 0 60 3 0 2和 3 0 3 71176)
河北省自然科学基金 (3 0 2 3 12 )资助。