摘要
Ginkgo biloba is one of most famous officinal plant in China. In the past, many varieties were selected and planted, but there is not still identification system for these varieties. This paper studied DNA fingerprints of the main G. biloba varieties cultivated all over the country using ISSR DNA marker. The results indicated ISSR marker was effective highly for identifying 13 varieties. Only using primer ISSR46 and ISSR44, 13 varieties were able to be identified based on 11 polymorphic loci (ISSR46-550,ISSR46-670,ISSR46-740,ISSR46-1000,ISSR46-1159,ISSR46-1359,ISSR46-1600,ISSR44-520,ISSR44-580,ISSR44-660,ISSR44-1750). In addition, we found ISSR marker was stable and repeatable highly compared with RAPD marker.
Ginkgo biloba is one of most famous officinal plant in China. In the past, many varieties were selected and planted, but there is not still identification system for these varieties. This paper studied DNA fingerprints of the main G. biloba varieties cultivated all over the country using ISSR DNA marker. The results indicated ISSR marker was effective highly for identifying 13 varieties. Only using primer ISSR46 and ISSR44, 13 varieties were able to be identified based on 11 polymorphic loci (ISSR46-550,ISSR46-670,ISSR46-740,ISSR46-1000,ISSR46-1159,ISSR46-1359,ISSR46-1600,ISSR44-520,ISSR44-580,ISSR44-660,ISSR44-1750). In addition, we found ISSR marker was stable and repeatable highly compared with RAPD marker.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2005年第1期202-204,共3页
Scientia Silvae Sinicae
