输卵管闭塞性不孕症患者宫颈解脲支原体DNA和沙眼衣原体DNA的荧光定量表达

Quantitative expressions of ureaplasma urealyticum DNA and chlamydia trachomatis DNA in cervical secretion in women with infertility due to tubal factor

目的 通过对输卵管性不孕症患者宫颈分泌物解脲支原体 (Ureaplasmaurealyticum ,UU)DNA和沙眼衣原体(Chlamydiatrachomatis ,CT)DNA的表达情况比较 ,探讨UU与输卵管性不孕症的相关性。方法 采用荧光定量PCR的方法 ,对4 3例输卵管性不孕症患者和 2 6例对照组的宫颈分泌物UU -DNA和CT -DNA进行同时检测。结果 (1)两组病例宫颈分泌物UU -DNA定量值 (拷贝数 )相近 ,而CT -DNA的定量值病例组比对照组高。 (2 )UU -DNA的检出率在两组对象中 ,宫颈分泌物的检出率分别为 5 8.1%和 5 0 .0 % ,差异均无统计学意义 (P >0 .0 5 ) ;但病例组宫颈CT -DNA的检出率 (2 5 .6 % )明显高于对照组 (3.8% ,P <0 .0 5 )。结论 UU可能不是输卵管性不孕症的主要致病菌 ,而CT感染与输卵管性不孕症则密切相关。

Objective Quantitative expressions of ureaplasma urealyticum(UU) DNA and chlamydia trachomatis (CT) DNA by TaqMan PCR in cervical secretions in women with tubal infertility,and to study the role of UU in tubal infertility.Methods From May 2001 to February 2002,43 women with tubal infertility were enrolled as test group and 26 inpatients who had no tubal pathology as control group. By TaqMan PCR, we assayed quantitative expressions of UU-DNA and CT-DNA in their cervical secretions at the same time.Results (1) The UU-DNA quantitative expressions in cervical secretion in the test group and the control group were similar.The CT-DNA expressions were higher in the test group than that in the control group. (2)The positive rate of UU-DNA in the test group was 58.1%,whereas that in the control group was 50.0%,the difference being not statistically significant. However, CT-DNA was positive in 25.6% of cervical secretions in the test group,significantly higher than 3.8% in the control group (P<0.05).Conclusion UU may not be a main microbiologic agent in tubal infertility, but CT may be one of major pathogenic microorganisms in tubal occlusion.