摘要
采用叶盘法将高赖氨酸蛋白基因导入到烟草中.GUS组织化学染色、PCR扩增、Southernblot分析表明, 该基因已经整合到烟草基因组中.测定 10株转基因烟草叶片赖氨酸含量,大部分植株有着明显的提高,最高幅度达到了49. 89%. 图 6参
In this experiment, the lysine-rich protein gene was transformed into tobacco by leaf disc via Agrobacterium tumefaciens LBA4404 as a vehicle. Histochemical staining for GUS activity, PCR and Southern blot demonstrated that lys was integrated into the genome of tobacco plants. Data analysis showed that lysine content in most of 10 transgenic plants was obviously improved and the content in 1 transgenic plant was increased by 49.89%. Fig 6, Ref 11
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2005年第1期32-35,共4页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金 (30070467)
福建省科技厅科技项目(2001Z012)
福建省博士后基金项目资助~~
关键词
转基因烟草
赖氨酸含量
提高
transgenic tobacco
lysine content
improvement
