高血压患者动脉平滑肌细胞培养的探讨

Exploration of cell culture for hypertension patient vascular smooth muscle cells

目的对高血压患者动脉平滑肌细胞培养方法进行探讨。方法运用植块贴壁法对高血压患者动脉平滑肌细胞进行体外培养,并用倒置显微镜进行形态学观察和用免疫组化对培养细胞进行鉴定。结果运用植块贴壁法成功培养了高血压患者动脉平滑肌细胞,培养的细胞呈典型"峰谷"样生长,免疫组化S-P法检测α-平滑肌肌动蛋白单克隆抗体(α-SMActin)表达,确认为人血管平滑肌细胞(hVSMC)。经传代培养至3-5代,细胞生长特性未见异常改变。结论植块贴壁法培养的高血压患者动脉平滑肌细胞生长稳定性好,且培养与纯化能同步进行。

Objective To explore the method of cell culture for vascular smooth muscle cells of hypertension patient Methods Plant attached method was applied for cell culture vascular smooth muscle cells of hypertension patient Morphology of the cells were observed by inverted microscope, and identified by immunohistochemical methods. Results The hypertension patient vascular smooth muscle cells were cultured by plant attached method successfully. The cultured human vascular smooth muscle cells(hVSMCs) grew showed typical 'hill and valley' pattern and were identified by the immunohistochemical S-P method (α-S MActin). The hVSMCs had no obvious changes of growth characteristics after 3-5 generations of the culture. Conclusion The hVSMCs may have a good stability through the plant attached method. The hVSMCs could also be cultured and purified simultaneously.