MMP-2在食管癌中的表达及其与血管生成的相关性

Correlation between expression of matrix metalloproteinase-2 and angiogenesis in esophageal carcinoma

目的 观察食管癌组织、癌旁中重度不典型增生组织和切缘正常组织中基质金属蛋白酶 2 (MMP 2 )的表达及其与微血管密度 (MVD)的关系 ,探讨MMP 2对食管癌血管生成的作用和意义。方法 应用SP法对 4 8例食管癌组织、17例癌旁中重度不典型增生组织和 12例切缘正常组织进行MMP 2和CD34免疫组化染色 ,检测癌组织、癌旁不典型增生组织和正常黏膜组织中MMP 2的表达和MVD ,并分析MMP 2的表达与MVD之间 ,以及它们与食管癌临床病理特征之间的关系。结果 食管癌组织中MMP 2的表达显著高于癌旁组织 ,癌旁组织MMP 2的表达亦明显高于切缘正常组织。食管癌和癌旁组织中MMP 2的表达 ,与肿瘤内及癌旁组织内的平均MVD呈正相关 ;MMP 2表达和肿瘤中MVD计数与食管癌的淋巴结转移密切相关。结论 MMP 2可能在食管癌的血管生成和侵袭转移中起重要作用 ,可能是食管癌的重要促血管生成因子之一。

Objective To study expression of MMP 2 in relation to microvessel density (MVD) in esophageal carcinoma. Methods Forty eight specimens of esophageal carcinoma (Ec) and 17 specimens of grade Ⅱ+Ⅲ epithelial dysplasia (Dy) close to the tumor and 12 specimens of normal tissue (Nt) along the incisional margin were examined by S P immunohistochemical staining with anti MMP 2 monoclonal antibody. An anti CD34 monoclonal antibody was used to show MVD. Results MMP 2 expression in Ec was remarkably higher than that in Dy, which was higher than that in Nt. MMP 2 expression in Ec and Dy was significantly correlated with MVD in the tumor and nearby tissue. MMP 2 expression and MVD in Ec significantly associated with lymph node metastasis. Conclusion Expression of MMP 2 plays an important role in angiogenesis and lymph node metastasis of esophageal cancer.