HPLC-ELSD测定三七药材及其制剂中三七皂苷R_1、人参皂苷Rg_1及Rb_1的含量

Determination of notoginsenoside R_1, ginsenoside Rg_1 and Rb_1in Radix Notog inseng and its preparation by HPLC-ELSD

目的 :用HPLC-ELSD测定三七药材及其制剂血塞通注射液中三七皂苷R1、人参皂苷Rg1及Rb1的含量。方法 :色谱柱填料为十八烷基健合硅胶 ,流动相为乙腈 水 ,梯度洗脱 ;漂移管温度 10 5℃ ,载气流速 2.9L·min-1。结果 :三七皂苷R1、人参皂苷Rg1及Rb1分别在 0.4 5 0 6～ 2.2 5 30 ,1.4 772～ 7 386 0 ,1 2 0 6. 6～ 6 .0 330 μg呈良好线性关系 ;药材中 3种成分的平均回收率分别为 97 1% (RSD 1.9% ) ,96 8% (RSD 2.0 % ) ,97 0 % (RSD 2.2 % ) ;血塞通注射液中分别为 98.7% (RSD 1.9% ) ,98.5 % (RSD 1.8% ) ,98.1% (RSD 1.4 % )。结论 :该方法简便、准确、分离效果好 ,无干扰 。

Objective: To establish the quantitative method of notoginsenoside R_1, ginsenoside Rg_1 and Rb_1 in Radix Notoginseng and its preparation Xuesaitong injection by HPLC-ELSD. Method: The column was packed with 5 μm Diamonsil C_(18) stationary phase. The mobile phase consisted of acetonitrile-water, eluted in gradient mode. The temperature of drift tube was 105 ℃ and the nebulizer nitrogen flow rate was 2.9 L·min^(-1). Result: The linear ranges of the three components were 0.456～2.25 μg, 1.47～7.38 μg and 1.20～6.03 μg respectively. The average recoveries of the three components in Radix Notoginseng were 97.1%(RSD 1.9%), 96.8%(RSD 2.0%), 97.0%(RSD 2.2%) respectively;in Xuesaitong Injection were 98.7%(RSD 1.9%), 98.5%(RSD 1.8%), 98.1%(RSD 1.4%) respectively. Conclusion: It was proved that the method was reliable, simple,and precise, that could be used for quality control.