远志增强未孕大鼠子宫平滑肌运动的机制研究

Different mechanisms Mediated the potentiation uterine smooth muscle caused by Polygala tenuifolia decoction progesterone in virginal rats

目的:探讨远志对未孕大鼠子宫平滑肌电活动及离体子宫平滑肌条运动的影响及其机制。方法:在体组随机选取成年未孕大鼠60只分为实验组(n=15)与5个机制组(n=9),实验时腹腔注射1次远志水煎剂溶液(以下简称“远志”),剂量为0.02,0.04,0.08 g·kg-13种浓度,等容量生理盐水作自身对照。腹腔注射1种阻断剂10min后再注射远志0.04 g·kg-1;离体组肌条在1 g的前负荷下温育,远志浓度为2×10-4 g·mL-1,记录30 min;分别使用不同的受体拮抗剂, 5 min后再加入远志。用Biolap 410生物机能仪记录远志对子宫平滑肌电活动及离体子宫平滑肌运动的影响。结果与结论: 远志对未孕大鼠子宫平滑肌的运动有增强作用。远志对未孕大鼠子宫肌电的增强作用可能主要通过L型Ca2+通道,α受体,H1受体发挥作用,与前列腺素合成和释放有关,平滑肌细胞膜上的M受体,并不参与介导其兴奋作用;而其对未孕大鼠离体子宫平滑肌收缩运动的增强作用,可能是通过L型 Ca2+通道、α受体、H1受体发挥作用,与前列腺素合成和释放及M受体无关。

Objective: To research the effects of Polygala tenuifolia decoction on myoelectric activity of uterine smooth muscle and contractile activity of uterine smooth muscle strips of virginal rats. Method: To record the effects of P. tenuifolia decoction on myoelectric activity of uterine smooth muscle and contractile activity of uterine smooth muscle strips of virginal rats with biolap 410 biological system. Five blocking agents were used to study their mechanims respetively. Result: Diffeerent dosages of water extract of P. tenuifolia ( 0.02,0.04,0.08 g·kg^(-1)), could significantly potentiate uterine myoelectric agtivity and contractile activity of virginal rats. Conclusion: The effect of P. tenuifolia on myoelectric activity of uterine smooth muscle and contractile activity of uterine smooth muscle strips in rats may be mainly associated with H1 receptor, receptor, L-voltage-dependant calcium channels or prostaglandin synthese,its nothing to M receptor.