脑脉泰胶囊对脑缺血再灌大鼠学习记忆功能及脑组织乙酰胆碱含量的影响

Effects of Naomaitai capsule on learning and memory and content of Ach in rat model produced by cerebral ischemia-reperfusion

目的:探讨脑脉泰胶囊对血管性痴呆(VD)大鼠学习记忆功能及脑组织乙酰胆碱(Ach)含量的影响。方法:通过采用夹闭双侧颈总动脉20min,再灌10min,再夹闭20min的方式制成大鼠VD模型,以水迷宫实验进行学习记忆能力测试;通过采用四血管阻断再灌注法制成大鼠VD模型,以碱性羟胺法测定脑组织Ach含量;并观察脑脉泰胶囊对模型大鼠翻正反射恢复时间的影响。结果:脑脉泰胶囊能明显提高模型大鼠的学习记忆成绩(P<0.05,P<0.01),明显提高脑组织Ach含量(P<0.05,P<0.01),缩短模型大鼠翻正反射恢复时间(P<0.01)。结论:脑脉泰胶囊可能通过提高Ach含量而改善痴呆大鼠的学习记忆能力。

Objective: To observe the effects of Naomaitai Capsule on the learning and memory abilities and the content of acetylcholine(Ach) in the brain tissue of the model rat. Method: One vasular demential(VD) model was produced by occlusion of bilateral common carotid arteries in rats with following steps: ischemia 20 minutes-reperfusion 10 minutes-ischemia 20 minutes. The learning and memory abilities were tested by water maze.The other VD model was formed by 4-vessel occlusion-reperfusion injury in rats. The time of right reflect recovery and the content of Ach in the brain tissue were determined. Result: Naomaitai Capsule can significantly improve the learning and memory abilities of the dementia rats(P<0.05,P<0.01),increase the content of Ach(P<0.05,P<0.01) and reduce the time of right reflect recovery(P<0.01). Conclusion: Naomaitai Capsule can improve the learning and memory abilities by increasing the content of Ach of the VD rats.