抗氧化剂ebselen对SHRsp冠状动脉eNOS蛋白表达的影响

Effects of antioxidant ebselen (PZ51) on eNOS protein expression of coronary artery in SHRsp

目的 :研究新型抗氧化剂含硒谷胱甘肽过氧化物酶模拟物PZ5 1对卒中易感型自发性高血压大鼠 (SHRsp)高血压发展的慢性过程中冠状动脉内皮型一氧化氮合成酶 (eNOS)表达的影响。方法 :2 2只8周龄SHRsp随机分为PZ5 1组和对照组 ,灌胃治疗6周。测量心脏重量比值、心脏结构 ;采用分光光度计测心肌组织匀浆一氧化氮 (NO)的浓度 ;免疫组化检测冠状动脉eNOS蛋白表达。结果 :PZ5 1组较对照组心肌组织匀浆NO浓度显著升高 (1.18± 0 .2 5vs 0 .72± 0 .18μmol·mg-1prot ,P <0 .0 5 )、冠状动脉内皮eNOS蛋白表达显著增加 (7.4 5± 2 .10vs 2 .77± 2 .0 7,P <0 .0 5 )。结论 :PZ5 1治疗 6周显著增加了SHRsp冠状动脉eNOS蛋白表达及心肌组织NO的水平。

AIM: To investigate the effects of new antioxidant, seleno Glutathione peroxidase PZ51 on endothelial nitric oxide synthase (eNOS) protein expression of coronary artery in Stroke Prone Spontaneously Hypertensive rat (SHRsp) during the chronic process of hypertension. METHODS: 22 SHRsp of 8 week old which were randomly divided into PZ51 group and control group were administered by gavage for six weeks. Heart weight ratio, heart structure, the level of nitric oxide (NO) of myocardium and eNOS protein of coronary artery was examined. RESULTS: Compared with the control group, PZ51 significantly increased NO of myocardium homogenate ( 1.18 ± 0.25 vs 0.72 ± 0.18 μmol·mg -1 prot, P< 0.05 ) and eNOS protein expression( 7.45 ± 2.10 vs 2.77 ± 2.07 ,P< 0.05 )of coronary artery. CONCLUSION: After 6 weeks administration of PZ51, the eNOS protein expression of coronary artery and NO level of myocardium in SHRsp significantly increased.