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不同危险分组ALL细胞PI3K/AKT磷酸化激活的初步研究 被引量：2

Preliminary Study on Constitutive Activation of Phosphatidyl- Inositide 3 Kinase/AKT (PI3K/AKT) in Patients with Acute Lymphoblastic Leukemia of Different Risk Classifications
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摘要 目的　观察磷脂酰肌醇 - 3激酶 /蛋白激酶通路 (PI3K/AKT)在不同危险分组的白血病细胞中磷酸化激活的程度 ,初步了解急性白血病细胞PI3K/AKT激活与急淋白血病危险分组的关系。方法　使用蛋白印记分析法(WesternBlot)检测正常健康儿童、初发白血病患儿、缓解后患儿以及难治性白血病患儿白血病骨髓细胞中PI3K/AKT磷酸化激活程度。结果　正常健康儿童骨髓单个核细胞中无PI3K/AKT磷酸化激活 ,10例初发患儿中 3例、10例缓解后患儿 1例有PI3K/AKT磷酸化激活 ,6例难治患儿中 4例有PI3K磷酸化激活。结论　PI - 3K/AKT信号传导通路激活与否在淋巴细胞白血病危险分组和疾病转归的生物学过程中可能起着较重要作用 ,需进一步深入研究。 Objective To investigate the constitutive activation of phosphatidyl-inositide 3 kinase/AKT (PI3K/AKT) in the patients with acute lymphoblastic leukemia (ALL) of different risk classifications.Methods Total-AKT (T-AKT) and phosphorylated AKT (P-AKT) were detected by means of Western blotting in healthy donors,patients with de novo acute lymphoblastic leukemia,patients under complete remission and patients with refractory disease.Results T-AKT was detected in all samples studied.No P-AKT was found in healthy donors.P-AKT was detected in only 1 among 10 cases of patients under remission,and in 3 among 10 cases of patients with de novo ALL.In patients of refractory leukemia,4 of 6 cases were found with constitutive activation of P-AKT.Conclusion Phosphatidyl-inositide 3 kinase/AKT (PI3K/AKT) signal transduction pathway may play an important role in the risk classification and the prognosis of ALL after treatment.
作者 梁爱斌 李莉 谢晓恬 江华 叶辉 赵金彩 GU Long-jun
机构地区 同济大学附属同济医院儿科 上海第二医科大学附属新华医院儿童医学中心
出处 《同济大学学报（医学版）》 CAS 2005年第1期52-55,共4页 Journal of Tongji University(Medical Science)
基金 国家自然科学青年基金资助项目 (3 0 10 0 2 0 3 )
关键词 急淋白血病 危险分组 磷脂酰肌醇-3激酶 激活 ALL risk classification PI3K/AKT activation
分类号 R72 [医药卫生—儿科]
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参考文献9

  • 1Colby-Graham MF, Chordas C. The childhood leukemias [J]. J Pediatr Nurs,2003, (18): 87 - 95.
  • 2Pui CH, Relling MV, Campana D, et al. Childhood acute lymphoblastic leukemia[J]. Rev Clin Exp Hematol, 2002,6(2) :161-80.
  • 3Xu Q Simpson, S E Scialla, T J Bagg, et al. Survival of acute myeloid leukemia cells requires PI3 kinase activation [J]. Blood,2003,102(3) :972 - 980.
  • 4West K A, Castillo SS, Dennis PA. Activation of the PI3K/Akt pathway and chemotherapeutic resistance[J].Drug resistance updates, 2002,5 (3): 234 - 248.
  • 5Damu T, Hitoshi Okada, Jurgen Ruland, et al. Akt is activated in response to an apoptosis signal[J]. J Biol Chem,2001,276(32) :30461 - 30466.
  • 6Ng SSW, Tsao MS, Nicklee T, et al. Wortmannin inhibits PKB/Akt phosphorylation and promotes gemcitabine-induced apoptosis in human pancreatic cancer cells[J]. Cancer research, 2001,7 (10): 3269 - 3275.
  • 7Jones RG,Parsons M,Bonnard M, et al.PKB regulates T lymphocyte survival,nuclear factor kB activation,and BclXL levels in vivo[J].J exp Med,2000,15(191):1721-1734.
  • 8Giomore AP, Valentijn AJ, Wang P, et al. Activation of Bad by therapeutic inhibition of epidermal growth factor receptor and transaction by insulin-like growth factor receptor[J]. J Biol Chem,2002,277(31) :27643 - 27650.
  • 9Testa JR, Ballacosa A. AKT plays a central role in tumorigenesis[J]. Proc Natl Acad Sci USA, 2001, 98 (20):10983 - 10985.

同被引文献21

  • 1Avenllino R,Romano S,Parasole R,et al.Rapamycin stimulates apoptosis of chlidhood acute lymphoblastic leukemia cells[J].Blood,2005,106(4):1400-1406.
  • 2Vorwerk P,Wex H,Hohmann B,et al.Expression of components of the IGF signalling system in childhood acute lymphoblastic leukaemia[J].Mol Pathol,2002,55(1):40-45.
  • 3Wei G,Twomey D,Lamb J,et al.Gene expression-based chemical genomics identifies rapamycin as a modulator of MCL1 and glucocorticoid resistance[J].Cancer Cell,2006,10(4):331-342.
  • 4Schrappe M,Reter A,Zimmermann M,et al.Long-term results of four consecutive trials in childhood ALL performed by the ALL-BFM study group from 1981 to 1995.Berlin-Frankfurt-Munster[J].Leukemia,2000,14(12):2205-2222.
  • 5Gao N,Flynn DC,Zhang Z,et al.G1 cell cycle progres-sion and the expression of G1 cyclins are regulated by PI3K/AKT/mTOR/p70S6K1 signaling in human ovarian cancer cells[J].Am J Physiol Cell Physiol,2004,287(2):C281-291.
  • 6Gao N,Zhang Z,Jiang BH,et al.Role of PI3K/AKT/mTOR signaling in the cell cycle progression of human prostate cancer[J].Biochem Biophys Res Commun,2003,310(4):1124-1132.
  • 7West KA,Castillo SS,Dennis PA.Activation of the PI3K/Akt pathway and chemotherapeutic resistance[J].Drug Resist Updat,2002,5(6):234-248.
  • 8Martelli AM,Tabellini G,Bortul R,et al.Involvement of the phosphoinositide 3-kinase/Akt signaling pathway in the resistance to therapeutic treatments of human leukemias[J].Histol Histopathol,2005,20(1):239-252.
  • 9McCubrey JA, Abrams SL, Ligresti G, et al. Involvement of p53 and Raf/MEK/ERK pathways in hematopoietic drug resistance [ J]. Leukemia, 2008, 22 ( 11 ) :2080-2090.
  • 10Irish JM, Koteeha N, Nolan GP. Mapping normal and cancer cell signalling net- works: towards single-cell proteomies [ J ]. Nat Rev Cancer, 2006,6(2) :146-155.

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同济大学学报（医学版）
2005年 第1期

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