泛发性脓疱性银屑病与HLA-DQB1等位基因的相关性研究

The Association of HLA-DQB1 Alleles and Generalized Pustular Psoriasis

目的探讨山东汉族人泛发性脓疱性银屑病(GPP)与HLA-DQB1等位基因的相关性。方法运用聚合酶链反应-序列特异性引物(PCR-SSP)法,对38例山东汉族人GPP与94例健康对照进行HLA-DQB1等位基因分型。结果GPP患者组HLA-DQB10201、0603基因频率较对照组显著升高(OR=8.10,Pc=0.005;OR=5.06,Pc=0.013),而HLA-DQB10604基因频率较对照组明显降低(OR=0.08,Pc=0.039)。原有寻常性银屑病病史的GPP与HLA-DQB10201、0603强相关(OR=32.31,Pc=0.005;OR=12.42,Pc=0.005);而原无寻常性银屑病病史的GPP与HLA-DQB10602相关(OR=5.60,Pc=0.039)。结论山东汉族GPP与HLA-DQB10201、0603等位基因高度关联,原有寻常性银屑病病史的GPP与原无寻常性银屑病病史的GPP具有遗传异质性。

Objectives To investigate the association between HLA-DQB1 alleles and Han Chinese with generalized pustular psoriasis (GPP) from Shandong. Methods The distributing frequencies of HLA-DQB1 alleles were detected with polymerase chain reaction-sequence specific primers (PCR-SSP) in 38 GPP patients and 94 healthy subjects from Shandong. Results ①The frequencies of HLA-DQB1*0201 and *0603 alleles increased significantly in GPP patients compared with those of the healthy controls(Pc = 0.005 and Pc = 0.013 , respectively), and the frequency of HLA- DQB1*0604 alleles decreased (OR = 0.08, Pc = 0.039). ②There were significant association between the GPP patients with a previous history of psoriasis vulgaris and HLA-DQB1*0201 and *0603 alleles (OR = 32.31, Pc = 0.005; OR = 12.42, Pc = 0.005, respectively), and between the GPP patients without the history and HLA-DQB1*0602 alleles (OR = 5.60, Pc = 0.039). Conclusions HLA-DQB1*0201 and *0603 alleles are significantly associated with the Chinese patients with GPP in Han population from Shandong. There is a significant heterogeneity in the GPP patients with and without the history of psoriasis vulgaris.