摘要
利用TRIZOL试剂快速提取法、异硫氰酸胍法和改良的SDS法提取不同发育时期水稻颖果的总RNA,并通 过凝胶电泳、紫外分光光度法检测提取获得的RNA样品的品质.研究结果表明,改良的SDS法提取的RNA具有28S rRNA和18SrRNA2条清晰的条带,且无降解.其D260/D280为1.84～1.97,具有较高的纯度.其他2种方法获得的RNA 品质较差,有降解和弥散现象.将改良的SDS法提取的RNA逆转录成cDNA,作为PCR扩增的模板,可获得清晰的约 1.7kb的目的条带.该法提取的RNA用于Northern杂交,可产生特异的杂交信号.这些结果进一步证明了改良的SDS 法提取的RNA具有很高的品质与纯度,可以满足下一步分子生物学研究的需要.
Total RNA is isolated from rice caryopses displaying different development status by using the methods of TRIZOL Regent, Guanidine Thiocyanate and improved SDS. The quality of total RNA is analyzed through gel electrophoresis and UV spectrometer. The results indicate that the RNA isolated by the improved SDS method shows clear bands of 28S rRNA and 18S rRNA, and the value of D 260 /D 280 is 1.84 to 1.97. RNA isolated by TRIZOL Regent and Guanidine Thiocyanate methods degrades and disperses in some degrees. RNA isolated by the improved SDS method is used for RT-PCR and a clear 1.7 kb band can be observed in agarose gel. It is also used for Northern blot, and shows specific signal. These results demonstrate that the quality and purity of the RNA obtained through the improved SDS method can meet the demands of molecular biology experiment.
出处
《北京师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第1期79-81,共3页
Journal of Beijing Normal University(Natural Science)
基金
北京师范大学青年基金资助项目
关键词
水稻
颖果
总RNA
提取
rice
caryopses
total RNA
isolate
