小麦TOM7蛋白类似基因的克隆与分析(英文)

Cloning and Characterization of TOM7-like Gene in Wheat

通过RT PCR技术分离了编码小麦线粒体蛋白转运酶TOM7亚基 (TranslocaseofOuterMitochondrialMembranesubunit 7)的cDNA ,并克隆了该基因编码区的基因组DNA ,将该基因命名为TaTOM7。TaTOM7是一个没有内含子的基因 ,其编码的多肽具有一个位于中部的疏水跨膜区和两个分别位于N 端和C 端的亲水区域。来源于真菌、动物和植物的TOM7类似蛋白在疏水跨膜区高度保守 ,而在亲水区变异很大。在系统发育树中 ,TOM 7类似蛋白可以按动物、植物和真菌分为 3个类群。小麦TaTOM 7在基因组中以寡拷贝形式存在 ,它的表达在Ms2近等基因系之间和不同组织之间存在差异。中国春缺体 四体及端二体系分析表明 ,TaTOM

The cDNA encoding TOM7 (translocase of outer mitochondrial membrane subunit 7) like protein in wheat was cloned through RT-PCR,and its genomic DNA fragment was subsequently cloned.This gene was tentatively designated as TaTOM7.It has no intron in the coding region and its product possesses one hydrophobic trans-membrane domain in the middle,and one hydrophilic domain in the N-terminal and C-terminal domain,respectively.The amino acid composition in the trans-membrane domain of the TaTOM7 subunit is highly conserved among plants,animals and fungi.According to the phylogenetic tree,TOM7-like proteins from different species can be classified into three groups,representing plants,animals and fungi respectively.TaTOM7 is a single- or oligo-copy gene in the wheat genome,displaying different expression levels between Ms2 near-isogenic lines in some tissues,suggesting a role of Ms2 in its expression.TaTOM7 was mapped to chromosome group three of wheat.