摘要
矮泰引 3的矮生性状受两对独立遗传的半矮秆基因控制 ,利用SSR标记将这两个矮秆基因分别定位到第1和第 4染色体上。等位性测交的结果表明 ,位于第 1染色体上的矮秆基因与sd1是等位的 ,所以仍然称其为sd1 ;而位于第 4染色体上的矮秆基因是一个新基因 ,暂命名为sdt 2。利用SSR标记将sd1定位于RM 2 97、RM 30 2和RM 2 1 2的同一侧 ,而与OSR3共分离 ,它们之间的位置关系可能是RM2 97 RM 30 2 RM2 1 2 OSR3 sd1 ,遗传距离分别为 4 7cM、0cM、0 8cM和 0cM ,这与sd1在第 1染色体长臂上的确切位置是基本一致的。利用已有的SSR标记和拓展的SSR标记将sdt2定位于SSR332、RM1 30 5和RM 5 6 33、RM30 7、RM 4 0 1之间 ,它们的排列位置可能是SSR332 RM 1 30 5 sdt2 RM 5 6 33 RM 30 7 RM4 0 1 ,它们之间的遗传距离分别为 1 1 6cM、3 8cM、0 4cM、0cM和 0 .4cM。
The rice dwarf variety Aitaiyin3 is derived from a semidwarf cultivar Taiyin1.Genetic analysis indicated that the dwarf phenotype in Aitaiyin3 is involved in two recessive loci.Using bulk analysis with SSR markers,the two loci were located on the chromosome 1 and chromosome 4,respectively.Moreover,the allelism test showed that the locus on the chromosome 1 is allelic to the semidwarf gene sd1,while the other one is a new locus,here was named as sdt2.Further molecular mapping showed that sd1 was linked with SSR markers in the order of RM297-RM302-RM212-OSR3-sd1 with genetic distances of 4.7 cM,0 cM,0.8 cM and 0 cM,respectively.The linkage relationship of sdt2 with five SSR markers was SSR332-RM1305-sdt2-RM5633-RM307-RM401 with genetic distances of 11.6 cM,3.8 cM,0.4 cM,0 cM and 0.4 cM,respectively.
基金
国家自然科学基金资助项目 (编号 :30 2 70 70 5 )
国家重点基础研究发展规划项目 (编号 :2 0 0 3CB1 1 430 3)~~
