摘要
目的 研究四氯化碳诱导的肝硬化大鼠肝脏内皮素(endothelin, ET)受体基因的表达,并探讨其与门静脉压力的关系。方法 采用逆转录多聚酶链反应(RT PCR)测定大鼠肝组织 ETA受体和ETB受体mRNA的表达水平, 并测量门静脉压力。结果 肝硬化大鼠肝组织 ETA受体(0 4245±0 0612 vs 0 2792±0 1115,P<0 05) 和 ETB受体(0 5984±0 1047 vs 0 2938±0 1399,P< 0 05) mRNA 的表达均较对照组均显著升高;肝硬化大鼠门静脉压力较正常大鼠明显升高(16 08±2 19 vs8 25±2 56 cmH2O,P<0 05),并且ETA受体mRNA的表达与门静脉压力显著正相关(r=0 8074,P<0 05)。结论 四氯化碳诱导的肝硬化大鼠肝组织ET受体 mRNA的表达显著升高,并且 ETA受体 mRNA的表达与门静脉压力显著正相关, 表明 ET受体的高表达在门静脉高压症的发病机制中起重要作用。
Objective To investigate the relationship between the expression of hepatic endothelin (ET) receptor mRNA and portal pressure in carbon tetrachloride (CCl 4) induced cirrhotic rats. Methods After the model of liver cirrhosis was established by intragastric administration of CCl 4 in male adult SD rats, the expression of hepatic ETA and ETB receptor mRNA was determined by RT PCR and the portal pressure measured. Results The expression levels of both ETA (0 4245±0 0612 vs 0 2792±0 1115, n=8, P<0 05) and ETB (0 5984±0 1047 vs 0 2938±0 1399, n=8, P<0 05) receptor mRNA were significantly higher in cirrhotic rats than in the controls. Portal pressure was also significantly increased in the cirrhotic rats as compared with the controls (16 08±2 19 vs 8 25±2 56 cmH 2O, P<0 05). ETA receptor mRNA (r=0 8074, P<0 05) was positively correlated to portal pressure. Conclusions The expression of hepatic ETA and ETB receptor mRNA is significantly increased in CCl 4 induced cirrhotic rats and ETA receptor mRNA is significantly correlated to portal pressure. These suggest that the overexpression of ETA receptor mRNA may play an important role in the pathogenesis of portal hypertension.
出处
《中华肝胆外科杂志》
CAS
CSCD
2005年第2期110-113,共4页
Chinese Journal of Hepatobiliary Surgery
基金
北京市卫生重点学科项目资助(1998 卫科扶字11号)
